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cd8a fitc  (Miltenyi Biotec)


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    Structured Review

    Miltenyi Biotec cd8a fitc
    Cd8a Fitc, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 95/100, based on 26 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd8+fitc/CD8-FITC/pmc12922663-329-16-26
    Average 95 stars, based on 26 article reviews
    cd8a fitc - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    Staining:

    Article Title: CRISPR-mediated generation of a tumor-associated antigen-deficient Raji platform to investigate antigen loss in CAR-T cell therapy
    Article Snippet: .. Staining was CD3-PE, CD4-FITC, CD8-FITC, CD14-APC, CD56-APC, CD19-APC, CD20-APC, CD22-APC (Miltenyi Biotec). ..

    Article Title: Inhibition of autophagy enhances the antitumor efficacy of T/CAR T cell against neuroblastoma.
    Article Snippet: .. For staining of surface markers, cells were incubated for 20 min at 4 °C with the following mAb: CD3-vioblue (Miltenyi Biotec), CD8-FITC (Miltenyi Biotec), 1A7 clone antibody [34] with subsequent secondary Ab anti-mouse PE. .. For the TCR staining, cells were incubated with APC-conjugated PRAME-specific Dextramer (Immudex) according to the manufacturer’s protocol.

    Article Title: SFV Replicon Vector Harbouring Porcine Epidemic Diarrhoea Virus Immunogens Delivered by Attenuated Salmonella Typhimurium Induces PEDV Neutralising Antibodies and Lactogenic Immunogenicity in BALB/c Mice.
    Article Snippet: Fc Block solution was prepared using anti-CD16 (Miltenyi Biotec, Bergisch Gladbach, Germany) in FACS buffer, and cells will be incubated for 15 min at 4 ◦C. .. The cells were then stained with a cocktail containing anti-mouse CD3a-PE (Miltenyi Biotec, Germany), CD8-FITC (Miltenyi Biotec, Germany), and CD4-perCP-vio700 (Miltenyi Biotec, Germany) and incubated for 30 min at 4 ◦C in the dark. .. The proportions of CD4+ and CD8+ T cell subpopulations were determined using a MACSQuant® analyser (Miltenyi Biotec, Germany).

    Article Title: Cemiplimab in Locally Advanced or Metastatic Secondary Angiosarcomas (CEMangio): A Phase II Clinical Trial and Biomarker Analyses
    Article Snippet: PBMCs were thawed and stained first with a fixable viability dye eFluor 780 (65-0865-12, eBioscience). .. After washing with autoMACS staining buffer (130-091-221, Miltenyi Biotec), cells were stained with the following antibodies: CD8 FITC (555366, BD Biosciences, RRID:AB_395769), TIM-3 PE (119704, BioLegend, RRID:AB_345377), CD20 PERCP (130-113-376, Miltenyi Biotec, RRID:AB_2726144), CD3 PE-Cy7 (25-0038-42, Invitrogen, RRID:AB_1582253), CD56 APC (130-113-305, Miltenyi Biotec, RRID:AB_2726084), CD4 APC-R700 (564975, BD Biosciences, RRID:AB_2744418), PD-1 BV421 (329920, BioLegend, RRID:AB_10960742), LAG-3 Super Bright 600 (63-2239-42, Invitrogen, RRID:AB_2688188), and PD-L1 BV711 (329722, BioLegend, RRID:AB_2565764). ..

    Article Title: SFV Replicon Vector Harbouring Porcine Epidemic Diarrhoea Virus Immunogens Delivered by Attenuated Salmonella Typhimurium Induces PEDV Neutralising Antibodies and Lactogenic Immunogenicity in BALB/c Mice
    Article Snippet: Fc Block solution was prepared using anti-CD16 (Miltenyi Biotec, Bergisch Gladbach, Germany) in FACS buffer, and cells will be incubated for 15 min at 4 °C. .. The cells were then stained with a cocktail containing anti-mouse CD3a-PE (Miltenyi Biotec, Germany), CD8-FITC (Miltenyi Biotec, Germany), and CD4-perCP-vio700 (Miltenyi Biotec, Germany) and incubated for 30 min at 4 °C in the dark. .. The proportions of CD4 + and CD8 + T cell subpopulations were determined using a MACSQuant ® analyser (Miltenyi Biotec, Germany).

    Article Title: Cemiplimab in locally advanced or metastatic secondary angiosarcomas (CEMangio): A phase II clinical trial and biomarker analyses
    Article Snippet: PBMCs were thawed and stained first with a fixable viability dye eFluor 780 (65-0865-12, eBioscience). .. After washing with automacs staining buffer (130-091-221, Miltenyi Biotech) cells were stained with the following antibodies: CD8 FITC (555366 BD Biosciences, RRID:AB_395769), TIM-3 PE (119704, Biolegend, RRID:AB_345377), CD20 PERCP (130-113-376, Miltenyi Biotech, RRID:AB_2726144), CD3 PE-Cy7 (25-0038-42, Invitrogen, RRID:AB_1582253), CD56 APC (130-113-305, Miltenyi Biotech, RRID:AB_2726084), CD4 APC-R700 (564975, BD Biosciences, RRID:AB_2744418), PD-1 BV421 (329920, Biolegend, RRID:AB_10960742) LAG-3 superbright 600 (63-2239-42, Invitrogen, RRID:AB_2688188) and PD-L1 BV711 (329722, Biolegend RRID:AB_2565764). ..

    Incubation:

    Article Title: Inhibition of autophagy enhances the antitumor efficacy of T/CAR T cell against neuroblastoma.
    Article Snippet: .. For staining of surface markers, cells were incubated for 20 min at 4 °C with the following mAb: CD3-vioblue (Miltenyi Biotec), CD8-FITC (Miltenyi Biotec), 1A7 clone antibody [34] with subsequent secondary Ab anti-mouse PE. .. For the TCR staining, cells were incubated with APC-conjugated PRAME-specific Dextramer (Immudex) according to the manufacturer’s protocol.

    Article Title: SFV Replicon Vector Harbouring Porcine Epidemic Diarrhoea Virus Immunogens Delivered by Attenuated Salmonella Typhimurium Induces PEDV Neutralising Antibodies and Lactogenic Immunogenicity in BALB/c Mice.
    Article Snippet: Fc Block solution was prepared using anti-CD16 (Miltenyi Biotec, Bergisch Gladbach, Germany) in FACS buffer, and cells will be incubated for 15 min at 4 ◦C. .. The cells were then stained with a cocktail containing anti-mouse CD3a-PE (Miltenyi Biotec, Germany), CD8-FITC (Miltenyi Biotec, Germany), and CD4-perCP-vio700 (Miltenyi Biotec, Germany) and incubated for 30 min at 4 ◦C in the dark. .. The proportions of CD4+ and CD8+ T cell subpopulations were determined using a MACSQuant® analyser (Miltenyi Biotec, Germany).

    Article Title: SFV Replicon Vector Harbouring Porcine Epidemic Diarrhoea Virus Immunogens Delivered by Attenuated Salmonella Typhimurium Induces PEDV Neutralising Antibodies and Lactogenic Immunogenicity in BALB/c Mice
    Article Snippet: Fc Block solution was prepared using anti-CD16 (Miltenyi Biotec, Bergisch Gladbach, Germany) in FACS buffer, and cells will be incubated for 15 min at 4 °C. .. The cells were then stained with a cocktail containing anti-mouse CD3a-PE (Miltenyi Biotec, Germany), CD8-FITC (Miltenyi Biotec, Germany), and CD4-perCP-vio700 (Miltenyi Biotec, Germany) and incubated for 30 min at 4 °C in the dark. .. The proportions of CD4 + and CD8 + T cell subpopulations were determined using a MACSQuant ® analyser (Miltenyi Biotec, Germany).

    Flow Cytometry:

    Article Title: A multipronged Tα1 reset of CD8 + T cell cytotoxicity against breast cancer.
    Article Snippet: Thymosin α1 (Tα1) is an endogenous thymic peptide that enhances immune competence through activation of T cells, dendritic cells, and innate immune pathways.. However, its direct impact on CD8 T cell–mediated antitumor immunity in breast cancer remains unclear.. In this study, CD8 T cells isolated from peripheral blood of ten healthy donors were cultured under unstimulated, CD3/CD28-stimulated, Tα1-treated, or exhaustion–rescue conditions to evaluate cytotoxic activity against MDA-MB-231 breast cancer cells and CD44 cancer stem-like cells (CD44 CSC-like cells).

    other:

    Article Title: Epigenomic profiling of immune cell subtypes reveals H3K27ac-marked stress signatures after long-duration spaceflight
    Article Snippet: CD56-PE-Vio770 (Miltenyi Biotec Bergisch Gladbach, Germany; 130–113-313, clone REA196), CD3-PE (Miltenyi Biotec Bergisch Gladbach, Germany; 130–113-139, clone REA613), CD4-VioBlue (Miltenyi Biotec Bergisch Gladbach, Germany; 130–114-534, clone REA623), CD8-FITC (Miltenyi Biotec Bergisch Gladbach, Germany; 130–110-677, clone REA734), CD45-APC (Miltenyi Biotec Bergisch Gladbach, Germany; 130–110-633, clone REA747) and H3K27ac Rabbit mAb (Cell Signaling Technology, #8173).



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    Image Search Results


    ( A ) RT-qPCR detection of chimeric RNA UBA1-CDK16 in myeloid cells, NK cells, CD4 + T cells, CD8 + T cells, and B cells sorted from human PBMCs ( n = 2). ( B ) RT-qPCR detection of chimeric RNA UBA1-CDK16 on days 0, 3, and 5 of CD34 + cells myeloid differentiation ( n = 2). ( C ) Experimental timeline for shRNA transduction of expanded CD34 + cells followed by myeloid differentiation. ( D ) The effect of UBA1-CDK16 knockdown by shUC1 and shUC2. Short hairpin green fluorescent protein (shGFP) was used as negative control ( n = 2). ( E ) The fluorescent intensity distribution of CD11b in CD34 + cells undergo myeloid differentiation upon UBA1-CDK16 knockdown. Gated-out single cells were used for fluorescence-activated cell sorting (FACS) analysis. ( F ) CD11b mRNA expression detected by RT-qPCR. RNA samples were extracted from day 5 of myeloid differentiated cells. ( n = 3). ( G ) Uniform manifold approximation and projection (UMAP) projection of single-cell transcriptomes from shUC1 and shGFP samples from a female donor, clustered into major blood cell types. Cell types were annotated on the basis of canonical marker gene expression. MEPs, megakaryocyte-erythroid progenitors.

    Journal: Science Advances

    Article Title: UBA1-CDK16 : A female-specific chimeric RNA emerging through evolution and involved in immune regulation

    doi: 10.1126/sciadv.adz9784

    Figure Lengend Snippet: ( A ) RT-qPCR detection of chimeric RNA UBA1-CDK16 in myeloid cells, NK cells, CD4 + T cells, CD8 + T cells, and B cells sorted from human PBMCs ( n = 2). ( B ) RT-qPCR detection of chimeric RNA UBA1-CDK16 on days 0, 3, and 5 of CD34 + cells myeloid differentiation ( n = 2). ( C ) Experimental timeline for shRNA transduction of expanded CD34 + cells followed by myeloid differentiation. ( D ) The effect of UBA1-CDK16 knockdown by shUC1 and shUC2. Short hairpin green fluorescent protein (shGFP) was used as negative control ( n = 2). ( E ) The fluorescent intensity distribution of CD11b in CD34 + cells undergo myeloid differentiation upon UBA1-CDK16 knockdown. Gated-out single cells were used for fluorescence-activated cell sorting (FACS) analysis. ( F ) CD11b mRNA expression detected by RT-qPCR. RNA samples were extracted from day 5 of myeloid differentiated cells. ( n = 3). ( G ) Uniform manifold approximation and projection (UMAP) projection of single-cell transcriptomes from shUC1 and shGFP samples from a female donor, clustered into major blood cell types. Cell types were annotated on the basis of canonical marker gene expression. MEPs, megakaryocyte-erythroid progenitors.

    Article Snippet: Antibodies were added to stain surface markers for 30 min at 4°C (CD3 phycoerythrin (PE), Life Technologies; CD8 fluorescein isothiocyanate (FITC), Life Technologies; CD11b AF700, Life Technologies CD19 PerCP-Cy5.5, BioLegend; C33 BV605, BioLegend; CD56 BV711, BD).

    Techniques: Quantitative RT-PCR, shRNA, Transduction, Knockdown, Negative Control, Fluorescence, FACS, Expressing, Single Cell, Marker, Gene Expression